All researchCASE STUDY / 04 · Diagnostics · Microfluidics
Ammonia & Urea Dual-analyte microfluidic biochemical measurement
An IoT-enabled PDMS–glass microfluidic device for simultaneous assessment of ammonia and urea in blood serum using the modified Berthelot reaction, an LED–photodiode readout and a NodeMCU interface.
DiagnosticsAmmoniaUreaLab-on-chip
Ammonia & Urea · Research figure / project visual
0.1879 mg/LAmmonia detection limit
21.4091 mg/LUrea detection limit
5 VDevice supply voltage
01 / CHALLENGE
Why this work matters.
Biochemical analyses can benefit from compact and sample-efficient integrated microfluidic approaches.
02 / APPROACH
How it works.
Modified Berthelot colorimetry is coupled to a PDMS–glass microchannel, a 700 nm LED–photodiode measurement unit, a transimpedance amplifier and ThingSpeak-enabled ESP8266 readout. Urease converts urea into detectable ammonia.
03 / EXPERIMENTAL WORKFLOW
From concept to measurement
STEP 01
Sample introduction
STEP 02
Microfluidic processing
STEP 03
Dual-analyte reaction
STEP 04
Optical readout
STEP 05
Concentration calibration
04 / RESEARCH CONTRIBUTION
Contribution & context.
Co-design, microfluidic experimentation, optical detection and analytical validation.